Broad-spectrum Antibacterial Agent for the Treatment of Contaminated Cell Cultures

Normocure™ is a broad-spectrum antibacterial agent highly effective against Gram-positive and Gram-negative bacteria. Therefore, cell cultures contaminated with bacteria from the environment, such as Staphylococcus species [1] and Achromobacter species [2], can be efficiently cured by Normocure™ treatment. Unlike most antibiotics used to treat cell cultures, such as Penicillin-Streptomycin, Normocure™ is also active against multidrug-resistant bacteria. Additionally, Normocure™ has been successfully used in primary cell cultures for the treatment of contaminated samples to safely obtain induced pluripotent stem cells [3].

Normocure™ is provided as a ready-to-use solution (50 mg/ml). Simply add to contaminated cell cultures at the recommended concentration (100 μg/ml) for 2 weeks. After 3 passages, the bacterial contamination is totally eliminated.

Note: Normocure™ is intended only for the elimination of bacteria in cell cultures. Do not use in humans.

References:

1. Mirjalili A, et al., 2005. Microbial contamination of cell cultures: a 2 years study. Biologicals. 33(2):81–85.
2. Gray JS. et al., 2010. Got black swimming dots in your cell culture? Identification of Achromobacter as a novel cell culture contaminant. Biologicals. 38(2):273-7.
3. Geuder. et al., 2021. A non-invasive method to generate induced pluripotent stem cells from primate urine. Scientific Reports. 11:3516.

Specifications

Product concentration: 50 mg/ml

Normocure™ can be added:
- directly to the bottle of culture medium : 1 ml of Normocure™ for a 500 ml bottle
- to the flask containing the cells : 10 μl in a 25 cm² flask containing 5 ml culture medium.

Working concentration of Normocure™:100 μg/ml.

Decontamination period: 2 weeks (3 passages)

Quality Control: Activity of Normocure™ is rigorously tested by physicochemical and microbiological assays.

Contents

Normocure™ is provided as a cell culture tested, sterile filtered red solution.

This product is available :

• ant-noc : 2 x 1 ml (50 mg/ml)

 Normocure™ is shipped at room temperature.

 Upon receipt, store at 4°C for short term storage or at -20°C for long term storage.

Description

Normocure™ contains three bactericidal components belonging to different antibiotic families.

They act by inhibiting the protein synthesis or disrupting membrane integrity.

Their targets are different and completely absent in eukaryotic cells.

The cytotoxicity of Normocure™ is low, however a slowdown of cell growth may be observed. At the end of the treatment, when Normocure™ is removed from the culture medium, the cells return rapidly to their normal growth rate.

HEK293 cells (3 105 cells/ml) were spiked with a mixture of Gram- non-fermenting bacilli (Pseudomonas aeruginosa, Alcaligenes xylosodans, Achromobacter sp. and Stenotrophomonas maltophilia) at the concentration of 105 colony forming units (cfu)/ml, and were then either left untreated, or treated with 100 U/ml penicillin and 100 µg/ml streptomycin or with 100 µg/ml Normocure™, After 4 days at 37°C, 5% CO2, the bacteria were quantified (cfu per ml).

如需購買或咨詢該產品技術問題，請聯系Invivogen一級代理商--欣博盛生物